Lee Luckeydoo, a doctoral student in Environmental
Science at The Ohio State University, monitors vegetation community development at the
WRSIS wetlands. Quadrats are placed in each zone of the wetland: open water, average water
line, and shore. Within these quadrats, species and their abundance are recorded
throughout the growing season. The WRSIS wetlands are being allowed to revegetate
passively, with the only area planted being the buffer area, above the wetland, for
erosion control. This revegetation options relies on the seed bank present on site, and
the delivery of seeds from other sources by wind, wildlife or other vectors.

Passive revegetation should encourage the establishment of species adapted to
local conditions which can also tolerate the conditions created by using agricultural
drainage as a water source. During 1998 species richness, or total number of species, was
51 at the Defiance location, 44 at the Van Wert location, and 32 at the Fulton location.
Less than half of the total number of species on each site were considered to be wetland
species by the National List of Species that occur in wetlands of Ohio. Time and
appropriate conditions for delivered seeds will play a large role in the successful
establishment of vegetation on these sites. Diversity of vegetation over time will be
evaluated with Simpson's and Shannon-Wiener diversity indices. In addition to surveys of extant vegetation, seed banks are being evaluated on
these sites. In 1998, a seed bank study was conducted on the Defiance site soils and found
seven additional wetland species in the seed bank. The basin of the wetland was modified
in early 1999 to create more mudflat conditions to encourage the growth and establishment
of these new species and the spread of established species. Future and ongoing plans for
the WRSIS wetlands include: (1) estimation of algal and vascular vegetation biomass, (2)
studies on seed and propagule delivery to the WRSIS sites, (3) comparison with reference
wetlands, and (4) continued vegetation surveys.Lee holding a quadrat measuring device. Vegetative surveys
were conducted in 1 m by 1 m quadrats. Picture taken at the Defiance County (DARA) WRSIS
site.

Seed bank study using germination method. Tub in the foreground is the flooded
treatment. Tub in the background is the mudflat treatment. Seedlings are grown from soil
taken from three zones in the wetland - open water, waterline, and shore. Purpose is to
estimate viable seeds in seedbank under simulated conditions. Study started in 1998 and
continues in 1999.

Photo of vegetation established at the Van Wert County WRSIS site, 1999. Sedges,
bulrushes and cattails growing in sedimentation zone encourage microbial reactions such as
denitrification.
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