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Zhongtang Yu
Assistant Professor Education: Discipline(s): Grants & Awards: Current Grants & Awards
Gastrointestinal Microbiology: - 21. The relationship between poultry litter and the intestinal microbial community profile in broilers. USDA/NRI. 2008-2010. - 20. Mechanisms coordinating protozoal growth and recycling: toward improved efficiency of protein usage in the rumen. USDA/NRI. 2008-2010. - 19. Proprietary research on molecular microbial ecology of human intestinal tracts. 2008-2009. - 18. Production of yeast probiotics from crude glycerol derived from soybean-based biodiesel production. Ohio Soy Council. 2009-2010.
Anaerobic Digestion and Anaerobic Microbiology: - 16. Development of a temperature-phased anaerobic digestion process for enhanced conversion of solids in livestock manure and food wastes to methane. DOT/NE SUN GRANT. 2008-2009. 15. Proprietary research on molecular microbial ecology of human intestinal tracts. PI, $72,700. 11/2007-10/2008. 14. Development of a temperature-phased anaerobic digestion process for enhanced conversion of solids in livestock manure and food wastes to methane. DOT/NE SUN GRANT. PI, $100,000. 12/2007-11/2009. 13. The relationship between poultry litter and the intestinal microbial community profile in broilers. USDA/NRI, Co-PI, $308,086. 3/2008-2/2011. 12. Comprehensive examination of intestinal microflora of turkeys. USDA/Midwest Poultry Research Program. PI, $76,180. 4/2006-3/2007. 11. Proprietary research 0n molecular microbial ecology of human intestinal tracts. PI, $82,700. 1/2007-12/2007. 10. Research on anaerobic digestion: Optimization and scalability of anaerobic digestion of mixed high strength food processing wastes for renewable biogas energy. APHIS. Co-PI, $532,762. 8/2005-7/2008. 09. Anaerobic microbiology: Population dynamics & metabolism in anaerobic digesters. DOE. Co-PI, $271,405. 8/2006-7/2008. 08. Biomass to Energy: Optimization of Anaerobic Digestion for Biological Conversion of Biomass to Renewable Energy. USDA/APHIS, Co-PI. $652,750.00. 10/2005-10/2006. 07. Proprietary research on molecular microbial ecology of human intestinal tracts. Co-PI, $117,700. 9/2005-6/2006. 06. Reduction of tetracycline resistance present in food-animal manures by composting and anaerobic digestion. The OARDC Research Enhancement Competitive Grants Program. $50,000. 5/2005-5/2007. 05. Proprietary research on molecular microbial ecology of infant gastrointestinal tract. Co-PI, $58,000. 12/2004-11/2005. 04. Molecular ecological characterization of intestinal microflora in turkey. JAAP Memorial Research Foundation. $10,000. 8/2004-7/2005. 03. Antibiotic use in beef product systems and consumption of beef in elderly Americans. USDA-CSREES, Co-PI, $388,842 (of a total ~$1,300,000). 8/2003-7/2007. 02. Protozoan ecology and its impact on nitrogen transaction in ruminants. USDA/NRI, Co-PI, $255,000, 10/2002-9/2006. 01. Proprietary research on molecular microbial ecology of gastrointestinal tract swine and poultry. Co-PI, $41,000. 7/2001-7/2002. Publications: 6. Yu, Z., M. Morrison, and F.L. Schanbacher (2009). Methane Biogas as a Renewable Fuel. In A. Vertes, N. Qureshi, H. Yukawa, and H. Blaschek (eds.), Biomass to Biofuel. Wiley & Sons. Hoboken, NJ. 5. Yu, Z. and M. Morrison (2009). The animal and human gut ecosystems. In L.A. Jaykus, H. Wang, and L. Schlesinger (eds.), Foodborne Microbes: Shaping the host ecosystem. ASM Press. USA. 4. Yu, Z. and M. Morrison (2007). Antimicrobial resistance genes in livestock production systems: concept and consequences. p.439-455. In X. Meng, L.P. Ren and Z.J. Cao (eds.) Herbivore nutrition for the development of efficient, safe and sustainable livestock production. China Agricultural University Press. 3. Neufeld, J.D., Z. Yu, W. Lam, and W.W. Mohn (2005). SARST, Serial Analysis of Ribosomal Sequence Tags. In G.G. Kowalchuk, F.J. de Bruijn, I.M. Head, A.D. Akkermans, and J.D. van Elsas, J.D. (eds.), Molecular Microbial Ecology Manual, 2nd Ed. Kluwer Academic Publisher, The Netherlands. 2. Yu, Z. and R. Forsberg (2005). Nucleic acid extraction, oligonucleotide probes and PCR methods. In H.P.S. Makkar and C.S. McSweeney (eds.), Methods in Gut Microbial Ecology for Ruminants. Springer, The Netherlands. 1. Firkins, J.L. and Z. Yu (2006) Characterization and quantification of microbes in ruminants. In K. Sejrsen, T. Hvelplund, and M.O. Nielsen (eds.), Ruminant nutrition and physiology. Wageningen Academic Publisher, The Netherlands.
Peer-reviewed articles Chen, J., F.C. Michel Jr., S. Sreevatsan, M. Morrison, and Z. Yu (2009). Occurrence and persistence of erythromycin resistance genes (erm) tetracycline resistance genes (tet) in waste treatment systems on swine farms. Environmental International, under review. Kang, S., S.E. Denman, M. Morrison, Z. Yu, and C.S. McSweeney (2009). An efficient RNA extraction method for the estimation of gut microbial diversity by PCR. Current Microbiology, in press. Firkins, J.L., S.K.R. Karnati, and Z. Yu (2008). Linking rumen function to animal response by application of metagenomics techniques. Australian Journal of Experimental Agriculture, 2008, 48:1-11. Ezer, A., E. Matalon, S. Jindou, I. Borovok, N. Atamna, Z. Yu, M. Morrison, E.A. Bayer, and R. Lamed (2008). Cell surface enzyme attachment is mediated by family 37 carbohydrate-binding modules, unique to Ruminococcus albus. Journal of Bacteriology, 190:8220-8222. Sharma, R., J. Chen, E. Topp, J. Yanke, F. Larney, T. McAllister, M. Morrison, and Z. Yu (2008). Detection and quantification of antimicrobial resistance during composting of bovine manure collected from animals administered sub-therapeutic antimicrobials in Southern Alberta. Journal of Environmental Quality, in press. Qutyan, M., Z. Yu, and G.B. Smith (2008). Succession of denitrifying bacteria and dcmA genes in a biological activated carbon community degrading dichloromethane. BMC Microbiology, in press. Yu, Z., R. García-González, and M. Morrison (2008). Evaluations of different hypervariable regions of archaeal rrs in profiling of methanogens by Archaea-specific PCR and denaturing gradient gel electrophoresis. Applied and Environmental Microbiolog, 74:889-893. Chen, J., F. Fluharty, M. Morrison, and Z. Yu (2008). Development of resistance to both macrolide-lincosamide-streptogramin B (MLSB) and tetracyclines concurrent with use of tylosin in beef cattle. Journal of Animal Science, 86:2385-2391. Anderson, K., Z. Yu, J. Chen, J. Jenkins, P. Courtney, and M. Morrison (2008). Analyses of Bifidobacterium, Lactobacillus, and total bacterial populations in healthy volunteers consuming calcium gluconate by denaturing gradient gel electrophoresis and real-time PCR. International Journal of Probiotics and Prebiotics, 3:31-36. Chen, J., Z. Yu, F.C. Michel, T. Wittum, and M. Morrison (2007). Development and application of real-time PCR assays for quantification of erm genes conferring resistance to macrolide-lincosamides-streptogramin B in livestock manure and manure management systems. Applied and Environmental Microbiology, 73:4407-4416. Rismani-Yazdi, H., A.D. Christy, B.A. Dehority, M. Morrison, Z. Yu, and O.H. Tuovinen (2007). Electricity generation from cellulose by rumen microorganisms in microbial fuel cells. Biotechnology and Bioengineering, 97(6):1398-1407. Manuzon, M.Y., S.E. Hanna, H. Luo, Z. Yu, W.J. Harper, and H.H. Wang (2007). Quantitative assessment of antibiotic resistance gene pool in cheese samples by real- time TaqMan® PCR. Applied and Environmental Microbiology, 73:1676-1677. Firkins, J.L., Z. Yu, and M. Morrison (2007). Ruminal nitrogen metabolism: perspectives for integration of microbiology and nutrition for dairy. Journal of Dairy Science, 90(E. Suppl.):E1–E16. Karnati, S.K.R., J.T. Sylvester, S.M. Noftsger, Z. Yu, N.R. St-Pierre, and J.L. Firkins (2007). Assessment of ruminal bacterial populations and protozoal generation time in cows fed different methionine sources. Journal of Dairy Science, 90:798–809. McSweeney, C.S., S.E. Denman, A.-D. G. Wright, and Z. Yu (2007). Application of recent DNA/RNA-based techniques in rumen ecology. Asian-Australian Journal of Animal Science, 20:283-291. Yu, Z., M. Yu, and M. Morrison (2006). Improved serial analysis of V1 ribosomal sequence tags (SARST-V1) to support examination of bacterial diversity and community composition. Environmental Microbiology, 8(4):603-611. Yu, Z., F. C. Michel, G. Hansen, T. Wittum, and M. Morrison (2005). Development and application of real-time PCR assays for quantification of genes encoding tetracycline resistance. Applied and Environmental Microbiology, 71(11):6926-6933. Larue, R., Z. Yu, V.A. Parisi, and M. Morrison (2005). Ribosomal intergenic spacer- and DGGE-based analyses of microbial consortia associated with liquid and particulate fractions of rumen digesta. Environmental Microbiology, 7(4):530-540. Sylvester, J.T., S.K.R. Karnati, Z. Yu, M. Morrison, and J.L. Firkins (2005). Evaluation of a real-time PCR assay for measuring the ruminal pool and duodenal flow of protozoal nitrogen. Journal of Dairy Science, 88:2083-2095. Sylvester, J.T., S.K.R. Karnati, Z. Yu, M. Morrison, and J.L. Firkins (2004). Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR. Journal of Nutrition, 134:3378-3384. Yu, Z. and M. Morrison (2004). Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Applied and Environmental Microbiology, 70(8):4800-4806. Yu, Z. and M. Morrison (2004). Improved extraction of PCR-quality community DNA from digesta and fecal samples. BioTechniques 36:808-812. Neufeld, J.D., Z. Yu, W. Lam, and W.W. Mohn (2004). Serial Analysis of Ribosomal Sequence Tags (SARST): a high-throughput method for profiling complex microbial communities. Environmental Microbiology, 6:131-144. Smith, N., Z. Yu, and W.W. Mohn (2003). Stability of the bacterial community in a pulp mill effluent treatment system during normal operation and a system shutdown. Water Research, 37(20):4873-4884. Eriksson, M., E. Sodersten, Z. Yu, G. Dalhammar, and W.W. Mohn (2003). Degradation of polycyclic aromatic hydrocarbons at low temperature under aerobic and nitrate- reducing conditions in enrichment cultures from northern soils. Applied and Environmental Microbiology, 69:275-284. Karnati, S.K.R., Z. Yu, J.T. Sylvester, B.A. Dehority, M. Morrison, and J.L. Firkins (2003). Specific PAR amplification of protozoan 18S rDNA sequences from DNA extracted from rumen samples of cows for ecological analysis of ruminal protozoa. Journal of Animal Science, 81:812-815. Yu, Z. and W.W. Mohn (2002). Bioaugmentation with the resin acid-degrading bacterium Zoogloea resiniphila DhA-35 to counteract pH stress in an aerated lagoon treating pulp and paper mill effluent. Water Research, 36:1793-2801. Thomassin-Lacroix, E.J., Z. Yu, M. Erikson, K.J. Reimer, and W.W. Mohn (2001). DNA- based and culture-based characterization of a hydrocarbon-degrading consortium enriched from arctic soil. Canadian Journal of Microbiology, 47:1107-1115. Muttray, A.F., Z. Yu, and W.W. Mohn (2001). Population dynamics and metabolic activity of Pseudomonas abietaniphila BKME-9 within pulp mill wastewater microbial communities assayed by competitive PCR and RT-PCR. FEMS Microbiology Ecology, 38:21-31. Ka, Jong-Ok, Z. Yu, and W.W. Mohn (2001). Monitoring the size and metabolic activity of the bacterial community during biostimulation of fuel-contaminated soil using competitive PCR and RT-PCR. Microbial Ecology, 42:267-273. Yu, Z. and W.W. Mohn (2001). Bacterial diversity and community structure in an aerated lagoon revealed by ribosomal intergenic spacers analyses and 16S-rDNA sequencing. Applied and Environmental Microbiology, 67:1565-1574. Yu, Z. and W.W. Mohn (2001). Bioaugmentation with resin acid-degrading bacteria enhances resin acid removal in sequencing batch reactors treating pulp mill effluents. Water Research, 35:883-890. Yu, Z., G.R. Steward, and W.W. Mohn (2000). Apparent contradiction: psychrotolerant bacteria from hydrocarbon-contaminated Arctic tundra soils that degrade diterpenoids synthesized by trees. Applied and Environmental Microbiology, 66:5148-5154. Yu, Z. and G.B. Smith (2000). Inhibition of methanogenesis by C1- and C2- polychlorinated aliphatic hydrocarbons. Environmental Toxicology and Chemistry, 19:2212–2217. Yu, Z. and G.B. Smith (2000). Dechlorination of polychlorinated methanes by a sequential methanogenic-denitrifying bioreactor system. Applied Microbiology and Biotechnology, 53:484-489. Mohn, W.W., Z. Yu, and A. Muttray (2000). Lessons learned from Sphingomonas species that degrade abietane diterpenoids. Journal of Industrial Microbiology and Biotechnology, 23:374-379. Mohn, W.W., V. Martin, and Z. Yu (1999). Biochemistry and ecology of resin acid biodegradation in pulp and paper mill effluent treatment systems. Water Science and Technology, 40:273-280. Martin, V., Z. Yu, and W.W. Mohn (1999). Recent advances in understanding resin acid biodegradation: microbial diversity and metabolism. Archives of Microbiology, 172:131- 138. Yu, Z. and W.W. Mohn (1999). Occurrence of two resin acid-degrading bacteria and a gene encoding resin acid biodegradation in pulp and paper mill effluent biotreatment systems assayed by PCR. Microbial Ecology, 38: 114-125. Yu, Z. and W.W. Mohn (1999). Isolation and characterization of thermophilic bacteria capable of degrading dehydroabietic acids. Canadian Journal of Microbiology, 45:513-520. Yu, Z., P. Sahota, and W.W. Mohn (1999). Enhancement of resin acid removal in sequencing batch reactors treating pulp mill effluents by bioaugmentation with resin acid-degrading bacteria. Proceedings of Annual Conference of Canadian Pulp and Paper Association, Parksville, Canada, pp. 61-68. Yu, Z. and W.W. Mohn (1999). Microbial population dynamics of resin acid-degrading bacteria in biotreatment systems of pulp mill effluents. Proceedings of Science and Practice: Sustaining the Boreal Fores, Edmonton, Canada, pp.282-287. Mohn, W.W., V. Martin, A. Muttray, and Z. Yu (1999). Peeking into the black box: resin acid-degrading microbial populations in treatment systems for pulp mill effluent. Proceedings of Science and Practice: Sustaining the Boreal Forest, Edmonton, Canada, pp.276-281. Yu, Z. and W.W. Mohn (1999). Killing two birds with one stone: simultaneous extraction of DNA and RNA from activated sludge biomass. Canadian Journal of Microbiology, 45:269-272. Mohn, W.W., A. Muttray, and Z. Yu (1998). Ecological studies of resin acid-degrading bacteria in biological treatment systems using molecular probes. Proceedings of 7th International Conference on Biotechnology in Pulp and Paper Industry, Vancouver, Canada, Vol. C. pp.223-226. Yu, Z. and G.B. Smith (1997). Chloroform dechlorination by a wastewater methanogenic consortium and cell extracts of Methanosarcina barkeri. Water Research, 31:1879-1886.
Species: Dr. Yu is interested in ecological studies of microorganisms inside (mainly the gastrointestinal track) and outside (the surrounding environments) of food animals that are important to animal nutrition and health. He is also interested in studies of the microbiota of human gastrointestinal tract. At community level, the genetic and the functional diversity in the rumen and gastrointestinal tracts of animals and humans is being investigated. At population level, the dynamics of particular species or guilds, such as Bifidobacterium, Lactobacillus, and clostridia, E. coli in the gastrointestinal tract of animals and humans, and methanogens and cellulolytic bacteria in the rumen is studied both qualitatively and quantitatively. The ultimate goal is to advance basic understanding of the microbiota in these habitats and to apply such knowledge to enhance feed utilization, to design more effective probiotics and prebiotics for animals and humans, and to minimize the environmental impact of food-animal production. His second research interest centers on the microbiological underpinning of anaerobic digestion of biomass wastes to methane biogas. Current projects are under way to define the phylogenetic and functional diversity and to examine the impact of feedstocks and operations on the microbial population dynamics and community structure in anaerobic digesters. The goal is to develop knowledge-based strategies to enhance anaerobic digestion efficiency and stability. He is also interested in the ecological studies of antibiotic resistance originating from food-animal production. These studies could advance our basic knowledge of the development and dissemination of antibiotic resistance from farms to the environments, providing us with useful information to help reduce the negative impact of antibiotic use in food animal production. In collaboration with Dr. Jeffery Firkins, ruminal protozoan populations are also being studied to better understand the roles that rumen protozoa play in ruminal protein metabolism. Both classical microbiological methods and molecular biology techniques (e.g., real-time PCR, recombinant DNA techniques, DGGE), metagenomics, comparative genomics, proteomics, and microarrays are employed in his research.
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110E Animal Sciences Building 614-292-3057 yu.226@osu.edu |