Bonello Site LogoPlant Pathology 704
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OSU Plant Pathology
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instructor: Pierluigi (Enrico) Bonello
office: 483C Kottman Hall
phone: 688-5401
e-mail: bonello.2@osu.edu
office hours: by appointment

classroom: 451 Kottman Hall
credit: 4 credit hours
lectures: TR, 10:30 - 11:48
laboratories: F, 2-5
prerequisites: Biology 114, Plant Pathology 300,
401, 660 or consent of instructor

Course Schedule:

Date
Lecture Number
Topic
Part 1: the small picture
April
2
1
Importance of fungi. The mycelium and other units and substrates for growth
4
2
Reproductive structures
5
Lab 1: lab techniques: sterile techniques, isolation and culturing of fungi, use of identification keys

9
3
Sexual reproduction
11
4
Asexual reproduction
12
Lab 2: Oomycetes ­ importance, identification, pathogenicity, biology and ecology of Pythium spp. and Phytophthora spp. (lab session in collaboration with Dr. Dorrance)

16
5
Fungal population biology I: sexual compatibility in fungi
18
6
Fungal population biology II: vegetative compatibility in fungi
19

Lab 3: Deuteromycetes ­ importance, identification, pathogenicity, biology and ecology of Verticillium spp.. (lab session in collaboration with Dr. Rowe)

23
7
Interactions with plants: anatomy, physiology, biochemistry (fungal toxins and plant defense chemicals, lecture by Dr. Graham)
25
8
Midterm exam (first 10 lectures)
26
Lab 4: Ascomycetes ­ importance, identification, pathogenicity, biology and ecology of Sclerotinia spp. and Ceratosystis spp. (lab session in collaboration with Dr. Rhodes)

30
9
Interactions with plants: gene-for-gene interactions
Part 2: the big picture
May
2
10
Molecular phylogeny (lecture by Dr. Kamoun). Species concept vs. concept of species complex in fungi
3
Lab 5: Basidiomycetes ­ importance, identification, pathogenicity, biology and ecology of rusts and smuts: Gymnosporangium spp. and Ustilago spp.

7
11
Life strategies of fungi
9
12
Structure of fungal communities I: development
10
Lab 6: presentation of molecular techniques; PCR amplification of ITS from mycelium

14
13
Fungal communities on herbaceous stems and grasses
16
14
Endophytic fungi
17
Lab 7: restriction enzyme digestion of internal transcribed spacer and molecular typing

21
15
Soil and rhizosphere fungi (Lecture by Dr. Serity Frey) Mycorrhizas
23
16
Paper discussion on soil and rhizospere fungi
24
Lab 8: Molecular phylogeny lab (lab session in collaboration with Dr. Kamoun)

28
17
Paper discussion on Mycorrhiza
30
18
Colonization of wood
31
Lab 9: field trip to collect ectomycorrhizas
June
4
19
Paper discussion on colonization of wood
6
20
Synthesis: role of fungi in ecosystem function
7
Lab 10: Presentation of monographs

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